Publications by authors named "J Fothergill"
With legalisation of cannabis for both medicinal and recreational use expanding to more world nations, grasping its effects on the human body is vital. The microbiome is critical to human health and disease, and accumulating data suggests that it is influenced by a variety of external variables, including marijuana/cannabis and cannabinoids. We therefore conducted a comprehensive assessment of the literature to analyse cannabis and cannabinoid effects on the human microbiota.
View Article and Find Full Text PDFTo combat the rising global issue of antibiotic resistance, the accelerated development of novel antibiotics is essential. Current preclinical antimicrobial development yields a significant number of leads that prove unsuitable either prior to or during clinical trials. To increase the efficiency of preclinical development, relevant, standardized, accessible, and cost-effective models must be developed.
View Article and Find Full Text PDFThe surge in multidrug-resistant bacteria against conventional antibiotics is a rapidly developing global health crisis necessitating novel infection management strategies. Host defence peptides (HDPs), also known as antimicrobial peptides (AMPs), offer a promising alternative to traditional antibiotics, but their practical translation is limited by their susceptibility to proteases and potential off-site cytotoxicity. In this paper, we investigate the feasibility of using gelatin emulsion gels (GELs), prepared using a water-in-oil (W/O) method, for the delivery of HDPs DJK-5 and IDR-1018 to improve their clinical utility.
View Article and Find Full Text PDFArticle Synopsis
- Ribosomal RNA gene amplicon sequencing is used to analyze microbiomes in health and disease, with Nanopore sequencing offering better species-level classification but needing optimized protocols.
- Different extraction methods (with and without bead-beating) were tested on a mock microbial community, revealing that bead-beating (MLB) provided the most diverse bacterial profiles and affected fungal composition significantly.
- The study suggests that simpler lysis conditions (ML) may be optimal for DNA yield and taxonomic accuracy, laying the groundwork for future combined bacterial and fungal microbiome research.
Objectives: Pseudomonas aeruginosa is the most common pathogen in the bronchiectasis lung, associated with worsened outcomes. P. aeruginosa genomic studies in this context have been limited to single-country, European studies.
View Article and Find Full Text PDF