Comparison of albumin excretion rate obtained with different times of collection.

This study was undertaken to assess the usefulness of different techniques for determination of albumin excretion rate (AER). Ninety patients with type I (insulin-dependent) diabetes mellitus and 45 with type II (non-insulin-dependent) diabetes mellitus, with AER/24 h of less than 200 micrograms/min, were included. All patients were free of major systemic complications of diabetes and overt kidney disease (mean serum creatinine 1.

Intestinal digestion of dipeptides in experimental diabetes in the rat.

Intestinal digestion of two dipeptides, leucyl-leucine and phenylalanyl glycine was studied in vivo in diabetic and control rats utilizing the segmental perfusion technique. Both proximal and distal segments of the small intestine were perfused with 20 mM of each substrate with 140 mM NaCl and 0.5% polyethylene glycol.

Effect of alloxan-induced diabetes on intestinal peptidases in the rat.

Intestinal transport of amino aicds, similar to sugar absorption, is enhanced in experimental diabetes. Because peptidases play a significant role in peptide digestion, we examined the effect of diabetes on intestinal peptidases. Leucyl-naphthylamidase and leucyl-glycine hydrolase (brush border peptidases) and prolyl-glycine hydrolase (cytosol peptidase) were assayed in the brush border and cytosol fraction in diabetic rats 7 days after alloxan administration.