Scaling up morphological differentiation of pangolin scales: Serial, ontogenetic and evolutionary variation.

Pangolins are the most heavily trafficked mammals in the world, largely because of the high demand for their keratinous scales from the traditional Chinese medicine market. While seizures of pangolin material are largely composed of isolated scales, efficient approaches to reach species-level identification are missing. This mostly originates from the lack of comparative studies on the shape of pangolin scales, resulting in knowledge gaps on the imbricated effects of serial, ontogenetic, and evolutionary variations.

Peptide mapping of recombinant human interferon-gamma by reversed-phase liquid chromatography with on-line identification by thermospray mass spectrometry and UV absorption spectrometry.

The detection and identification of minor peaks in a complex peptide map of recombinant human interferon-gamma was realized by on-line analysis of the eluted peptides using thermospray mass spectrometry and UV absorbance spectrometry. By this procedure the time-consuming process of collection, purification and chemical sequence analysis is avoided. Owing to the formation of multiple charged ions, the domain of the covered masses is extended.

[Paradoxic effect of anti-T-2 toxin antibodies in the curative treatment of acute poisoning in mice].

We have obtained polyclonal anti-T-2 toxin antibodies with high affinity to T-2 toxin (K = 0.34 x 10(9) l/M at 4 degrees C), crossreacting with HT-2 toxin, one of the major metabolites. We have assessed these antibodies in prevention and therapy of the acute intoxication.

Pharmacokinetic equivalence of 5(ethyl(2H)5)- and unlabelled phenobarbitone.

The present study shows the absence of in vivo pharmacokinetic isotope effect on phenobarbitone (PB) C5-ethyl deuteration (PBd5) following oral administration to man of equimolar PB/PBd5 mixtures (0.40 mmol each). Plasma PB and PBd5 (17 days) and urine PB, PBd5 and parahydroxy-metabolites (PBOH, PBHOd5) levels were determined by GC-MS.

Study of isotope effects on protein binding by gas chromatography/mass spectrometry of theophylline-phenobarbitone and 2H, 13C, 15N isotopomers.

We describe a comparative study of human serum albumin (HSA) binding by equilibrium dialysis (pH 7.4, 37 degrees C, 3 h) for two groups of isotopic analogues: theophylline and 1-C(2H3)theophylline; unlabelled, 5(ethyl(2H5],-5(phenyl(2H5] and 1,3-15N;2-13C-phenobarbitone. Bound and free drug fractions are quantified by combined gas chromatography/mass spectrometry.