Deep and Superficial Dyspareunia Questionnaire: a patient-reported outcome measure for genito-pelvic dyspareunia.

Introduction: Dyspareunia affects 8%-22% of women worldwide and an unknown number of gender-diverse people. Dyspareunia is commonly categorized into deep and superficial subtypes based on pain location and underlying etiology; however, current assessment tools inadequately differentiate between pain locations.

Aim: This study aimed to develop a patient-reported outcome measure (PROM) that independently assesses deep and superficial dyspareunia and its psychosocial correlates: the Deep and Superficial Dyspareunia Questionnaire (DSDQ).

Genome-wide analyses of Mycobacterium tuberculosis complex isolates reveal insights into circulating lineages and drug resistance mutations in The Gambia.

Tuberculosis (TB), caused by the complex (MTBC), remains a pressing global health challenge, with the West African region, including The Gambia, experiencing a substantial burden. This study explores the genetic diversity of MTBC strains circulating in The Gambia for nearly two decades (2002-2021) to enhance understanding of drug resistance dynamics and inform targeted diagnostic and treatment strategies. Using whole-genome sequencing (WGS) data from 1,803 TB isolates, we identified the predominance of lineage 4 (L4, 67.

An Industry Perspective on the Use of Novel Excipients in Lipid Nanoparticles-Nonclinical Considerations.

Nucleic acid drug delivery with lipid nanoparticle (LNP) formulations has enabled the development of novel therapeutics and vaccines. LNP formulations are composed of both naturally occurring and synthetic lipid excipients. This perspective shares current practices in the nonclinical safety assessment of novel lipid excipients contained in LNP formulations and identifies gaps in current regulatory guidance on this topic.

Does Productivity in Healthcare Matter? An Unfortunate Post-Pandemic Legacy.

In speeches this year, a bank of canada official announced that our country experienced no growth in productivity over the past two years (Rogers 2024a, 2024b). To improve this predicament, the bank says businesses should focus on adding value and being more efficient. These recommendations should be heeded by healthcare - the industry exceeds 12% of Canada's gross domestic product and is growing (CIHI 2024b).

Cullin-RING ligase BioE3 reveals molecular-glue-induced neosubstrates and rewiring of the endogenous Cereblon ubiquitome.

Background: The specificity of the ubiquitination process is mediated by the E3 ligases. Discriminating genuine substrates of E3s from mere interacting proteins is one of the major challenges in the field. We previously developed BioE3, a biotin-based approach that uses BirA-E3 fusions together with ubiquitin fused to a low-affinity AviTag to obtain a site-specific and proximity-dependent biotinylation of the substrates.