Publications by authors named "J F Goossens"
Primary human mast cells (MC) obtained through culturing of blood-derived MC progenitors are the preferred model for the study of MRGPRX2- IgE-mediated MC activation. In order to assess the impact of culture conditions on functional MRGPRX2 expression, we cultured CD34-enriched PBMC from peripheral whole blood (PB) and buffy coat (BC) samples in MethoCult medium containing stem cell factor (SCF) and interleukin (IL)-3, modified through variations in seeding density and adding or withholding IL-6, IL-9 and fetal bovine serum (FBS). Functional expression of MRGPRX2 was assessed after 4 weeks via flow cytometry.
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- Intense physical exercise in adolescents can lead to exercise-induced bronchoconstriction (EIB), which affects 22% of athletes studied, with many being unaware of their condition.
- The study involved 327 adolescent athletes who underwent various tests, revealing links between EIB, asthma history, and atopy, with many EIB athletes not using inhalation therapy.
- Effective screening methods like questionnaires and lung function tests were highlighted, emphasizing the need for better detection of EIB, particularly in athletes exposed to air pollution.
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- The study investigates the relationship between traumatic brain injury (TBI) and Alzheimer's disease (AD) by comparing AD patients with and without a history of TBI, focusing on various biomarkers in their cerebrospinal fluid (CSF).
- Researchers found no significant differences in baseline CSF biomarker levels or cognitive decline between the two groups of AD patients, suggesting that TBI may not directly affect AD progression.
- However, TBI occurring more than five years prior was linked to higher levels of specific biomarkers (NPTX2 and a trend for SNAP25), indicating possible long-term synaptic dysfunction effects when TBI occurs before the onset of AD.
Emerging pollutants derived from human and animal sources, are present in soils and pose significant environmental and health impacts, even at low concentrations. Their detection in soil is analytically complex due to soil interference and the rapid degradation of compounds in the matrix. In this study, a protocol was optimized for quantifying hormonal steroids ( = 7), human drugs ( = 3), and antibiotics ( = 3) by a dual-phase extraction using QuEChERS and Solid Phase Extraction (SPE), followed by analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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