Publications by authors named "J F Dynes"
In Alzheimer's disease (AD), amyloid-beta (Aβ) peptides are produced by proteolytic cleavage of the amyloid precursor protein (APP), which can occur during synaptic vesicle (SV) cycling at presynapses. Precisely how amyloidogenic APP processing may impair presynaptic proteostasis and how to therapeutically target this process remains poorly understood. Using knock-in mouse models of early Aβ pathology, we found proteins with hampered degradation accumulate at presynaptic sites.
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- - Cu nanoparticles (NPs) are important for various applications, but traditional methods of making them often lead to problems like surface passivation and clumping, which hurt their performance.
- - The study introduces a new method of embedding Cu NPs on a specific support that improves their stability and function by enhancing interactions with the support and preventing typical issues like oxidation and agglomeration.
- - It finds that these modified Cu NPs react differently with carbon monoxide (CO) than standard Cu NPs, thanks to a unique electronic structure that allows for a more efficient reaction mechanism.
Photoswitching alters fluorescence readout of jGCaMP8 Ca indicators tethered to Orai1 channels.
Proc Natl Acad Sci U S A
September 2023
We used electrophysiology and Ca channel tethering to evaluate the performance of jGCaMP8 genetically encoded Ca indicators (GECIs). Orai1 Ca channel-jGCaMP8 fusions were transfected into HEK 293A cells and jGCaMP8 fluorescence responses recorded by simultaneous total internal reflection fluorescence microscopy and whole-cell patch clamp electrophysiology. Noninactivating currents from the Orai1 Y80E mutant provided a steady flux of Ca controlled on a millisecond time scale by step changes in membrane potential.
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- The study examines how de-methyl esterification of homogalacturonan, combined with calcium cross-linking, may improve freezing survival in cold-acclimated plants by making their cell walls less porous.
- Japanese bunching onion leaves showed an 8 °C increase in freezing survival and a significant reduction in tissue permeability after two weeks of cold acclimation, linked to higher pectin methylesterase activity and lower homogalacturonan methylation.
- Treatment with calcium chloride in non-acclimated plants increased certain cell wall components and decreased visible pores, but did not enhance survival to freezing; rather, it aided in ice nucleation reduction to levels seen in cold-acclimated plants.*
The electroreduction reaction of CO (ECORR) requires high-performance catalysts to convert CO into useful chemicals. Transition metal-based atomically dispersed catalysts are promising for the high selectivity and activity in ECORR. This work presents a series of atomically dispersed Co, Fe bimetallic catalysts by carbonizing the Fe-introduced Co-zeolitic-imidazolate-framework (C-Fe-Co-ZIF) for the syngas generation from ECORR.
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