The application of whole-cell protein electrophoresis for the classification and identification of basidiomycetous yeast species.

The relationships among 65 basidiomycetous yeast strains were determined by one-dimensional electrophoresis of SDS-solubilized whole-cell proteins. Protein profiles were compared by the Pearson product moment correlation coefficient (r). The strains investigated represented species from the genera Cystofilobasidium, Filobasidium, Filobasidiella, Kondoa, Leucosporidium, Mrakia and Rhodosporidium.

Cervical epidural anesthesia for head and neck surgery.

Patients with head and neck cancer frequently suffer from chronic obstructive pulmonary disease and arteriosclerotic cardiovascular disease, making them poor risks for anesthesia. Arrhythmogenic inhalation anesthetics along with assisted ventilation used in general anesthesia further complicate the intraoperative, as well as the postoperative, course. Cervical epidural anesthesia has been employed in a number of cases of breast and upper thoracic surgery at Walter Reed Army Medical Center with remarkable success.

Nociceptive flexion reflexes elicited by noxious laser radiant heat in man.

This study, carried out in humans, is an attempt to elicit nociceptive flexion reflexes and to investigate the sensation reported by the subjects, using thermal stimulation achieved with laser radiant heat. It was found that noxious termal stimulation elicited long latency nociceptive reflexes very well correlated with a pricking pain. Since thermal stimulus is more specific and natural than an electrical one, it seems that this method should be of interest for pain research in man.

Experimental trabeculotomy with a monomode argon laser.

The laser trabeculotomy is one of the new techniques using light as a surgical ability in the treatment of some ocular diseases. The trabecula, in fact, plays an important part in the absorption of the aqueous humor and its bad functioning is the reason for chronic glaucoma. Here are some results obtained on in vitro trabecula with YAG-neodymium laser and an argon laser, thus showing that it is necessary to have very good focussing if we want to obtain a drill on the trabecula without having an excessive laser power.