Publications by authors named "J Eugene Lammers"
Vitrification has revolutionized embryo cryopreservation, but represents a significant workload in the IVF lab. We evaluated here an ultrafast blastocyst warming procedure in order to improve workflow while maintaining clinical outcome. We first evaluated the expression of main markers of lineage specification in a subset of blastocysts donated to research warmed with ultrafast protocol.
View Article and Find Full Text PDFRigidified nucleoside derivatives with (N)-methanocarba replacement of ribose have been repurposed as peripheral subtype-selective 5-HT serotonin receptor antagonists for heart and lung fibrosis and intestinal/vascular conditions. 4'-Cyano derivative (MRS8209; , 4.27 nM) was 47-fold (human binding, but not rat and mouse) and 724-fold (functionally) selective at 5-HTR, compared to antitarget 5-HTR, and predicted to form a stable receptor complex using docking and molecular dynamics.
View Article and Find Full Text PDFArticle Synopsis
- XIST long noncoding RNA plays a key role in X chromosome inactivation in placental mammals but is present on both X chromosomes in early human embryos without silencing them.
- XACT lncRNA accumulates alongside XIST on active X chromosomes and may counteract XIST's functions.
- Research using human embryonic stem cells reveals that XIST modifies chromatin and reduces transcription of X-linked genes, while XACT's absence does not significantly impact XIST’s activity or gene expression, indicating that XIST has a role prior to XCI and highlights a mechanism of temporary X chromosome dosage compensation.
DNA-protein crosslinks (DPCs) arise from enzymatic intermediates, metabolism or chemicals like chemotherapeutics. DPCs are highly cytotoxic as they impede DNA-based processes such as replication, which is counteracted through proteolysis-mediated DPC removal by spartan (SPRTN) or the proteasome. However, whether DPCs affect transcription and how transcription-blocking DPCs are repaired remains largely unknown.
View Article and Find Full Text PDFEvaluation of predictive factors of septic wrist to avoid overdiagnosis.
J Plast Reconstr Aesthet Surg
May 2024
Background: The existing diagnostic criteria for septic wrist are nonspecific, exposing patients with noninfectious etiologies to surgical morbidity. This study aimed to identify predictors differentiating septic wrist from other etiologies.
Methods: An institutional review board-approved retrospective review was conducted on patients with a presumed diagnosis of septic wrist (2003-2022).