High-resolution 1H NMR study of membrane phospholipids in rat pancreas stimulated by caerulein.

In this study, the peak observed at 3.2 +/- 0.2 ppm in the 1H nuclear magnetic resonance (NMR) spectra recorded at 60 MHz of rat pancreas stimulated by caerulein was characterized at higher resolution (100 and 300 MHz).

Combined magnetic resonance imaging and spectrometry for characterization of human pancreatic adenocarcinoma (Capan-1) heterotransplanted in nude mice.

Human pancreatic cancer cells (Capan-1 cell line) were heterotransplanted subcutaneously into nude mice. The resulting macroscopically visible tumours were analyzed by proton nuclear magnetic resonance spectrometry in a search for characteristic parameters. The Capan-1 xenografts were visualized by nuclear magnetic resonance imaging and were characterized by a significantly increased transverse relaxation time T2 (125 +/- 32 ms) compared with that recorded from ex vivo healthy human pancreatic tissue (60 +/- 11 ms).

Characterization of a specific signal from human pancreatic tumors heterotransplanted into nude mice. Study by high resolution 1H NMR and HPLC.

In a previous study, we demonstrated the existence of a 3.2 +/- 0.2 ppm peak in the 1H NMR spectrum at 60 MHz from human pancreatic adenocarcinomas (Capan-1 cell) heterotransplanted into nude mice.

Creatine phosphate as energy source in the cerulein-stimulated rat pancreas study by 31P nuclear magnetic resonance.

Stimulation of the rat exocrine pancreas by cerulein induces a variety of cellular processes, some of which require the expenditure of energy. In this study, changes in the amounts of various energy metabolites, including creatine phosphate (PCr), ATP, and ADP were determined by high-resolution 31P NMR spectroscopy. The spectrum of a perchloric acid extract of pancreas from the 48 h fasted rat was taken as a reference for comparison of 31P NMR spectra recorded after stimulation by cerulein.

Demonstration of a new resonance peak by proton NMR in rat pancreas stimulated with caerulein.

Low-resolution proton nuclear magnetic resonance (1H NMR) analysis of the pancreas of rats stimulated with caerulein, a cholecystokinin analog, was found to differ from that of control rats either fed ad libitum or fasted for two days. The hormonal stimulation induced (a) an increase in the longitudinal relaxation time T1; (b) a 1H NMR peak situated at 1.8 +/- 0.