Publications by authors named "J E Smitz"
The aims of this study were to evaluate the doxorubicin concentration that induces toxic effects on culture of isolated mouse secondary follicles and to investigate whether resveratrol can inhibit or reduce this toxicity. Secondary follicles were isolated and cultured for 12 days in control medium (α-MEM) or in α-MEM supplemented with doxorubicin (0.1 µg/ml) or different concentrations of resveratrol (0.
View Article and Find Full Text PDFObjective: To compare oocyte maturation rates and pregnancy outcomes in women with polycystic ovary syndrome (PCOS) undergoing biphasic in vitro maturation (capacitation in vitro maturation [CAPA-IVM]) with vs. without follicle-stimulating hormone (FSH) priming.
Design: Randomized, controlled, assessor-blinded trial.
Purpose: This study investigated the differences in the maturation rate of single versus grouped cumulus-oocyte complexes (COCs) culture methods for capacitation in vitro maturation (CAPA-IVM) in women with polycystic ovary syndrome (PCOS).
Methods: This study was performed at My Duc Phu Nhuan Hospital, Vietnam from October 1, 2020 to October 24, 2021. Women aged 18-37 years with a diagnosis of PCOS were recruited.
Research Question: Can a strategy for scoring oocyte quality, based on cumulus cell (CC) gene expression, prioritize oocytes with the highest implantation potential, while limiting the number of embryos to be processed in culture and the number of supernumerary embryos to be vitrified?
Design: An interventional, blinded, prospective cohort study was retrospectively analyzed. In the original study, patients underwent a fresh Day3 single embryo transfer with embryos ranked based on morphology and CC gene expression (Aurora Test). The additional ranking of the embryos with the Aurora Test resulted in significant higher clinical pregnancy and live birth rates.
Article Synopsis
- The study investigates the global DNA methylation differences in neonates conceived through two methods: capacitation-IVM (CAPA-IVM) and conventional IVF.
- Biological samples from both groups were collected at delivery, primarily focusing on placental tissue, cord blood, and buccal smears, analyzed for methylation using advanced techniques.
- Results indicate that while there were no significant differences in neonatal buccal smears, CAPA-IVM was linked to specific changes in DNA methylation patterns in placental tissue and showed variability in cord blood compared to conventional IVF, but without changes in gene expression.