Publications by authors named "J E Salk"
Polycyclic aromatic hydrocarbons (PAHs) are common environmental pollutants that originate from the incomplete combustion of organic materials. We investigated the clastogenicity and mutagenicity of benzo[]fluoranthene (BbF), one of 16 priority PAHs, in MutaMouse males after a 28 day oral exposure. BbF causes robust dose-dependent increases in micronucleus frequency in peripheral blood, indicative of chromosome damage.
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- After allogeneic hematopoietic cell transplantation (HCT), only a small fraction of donor stem cells help reconstitute the recipient's blood system, while the donor maintains a nearly normal stem cell pool.
- Researchers studied blood samples from 16 donor-recipient pairs, focusing on potential clonal hematopoiesis (CH) variants that could arise due to extra stress on donor cells post-transplant.
- Results showed similar mutation rates in both donors and recipients, with a small percentage of shared variants showing a significant increase in recipients over time, indicating that the human hematopoietic system has strong regenerative abilities even many years after HCT.
Article Synopsis
- De novo mutations (DNMs) are crucial in understanding genetic disorders but studying them is challenging due to technology limits in detecting rare mutations.
- Duplex Sequencing (DS) offers a highly accurate method, with a detection error of less than 1 in a billion base pairs, and was applied in this study to analyze DNA from blood and sperm of healthy young men.
- The results show a higher frequency of mutations in sperm than in blood, highlighting DS's capability to identify and quantify DNMs, which could help in understanding genetic diseases and hereditary risks.
Unlabelled: Polycyclic aromatic hydrocarbons (PAHs) are common environmental pollutants that originate from the incomplete combustion of organic materials. We investigated the clastogenicity and mutagenicity of benzo[ ]fluoranthene (BbF), one of 16 priority PAHs, in MutaMouse males after a 28-day oral exposure. BbF causes robust dose-dependent increases in micronucleus frequency in peripheral blood, indicative of chromosome damage.
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