Publications by authors named "J E Perilla"
Article Synopsis
- HIV-1 inhibitors Bevirimat (BVM) and Lenacapavir (LEN) work by blocking the production and maturation of infectious viral particles, but their exact mechanisms were not well understood due to limited structural data.
- Using perforated virus-like particles from mammalian cells, researchers utilized cryo-electron microscopy to achieve high-resolution structures of HIV-1 with BVM and LEN, marking the first detailed view of these inhibitors interacting with the immature virus.
- The study revealed how BVM binds to the Gag lattice and showed that LEN not only targets the mature capsid but also the immature lattice, causing significant changes in the capsid protein and Gag structure, which might influence the virus's maturation process.
Allosteric HIV-1 integrase (IN) inhibitors (ALLINIs) are investigational antiretroviral agents that potently impair virion maturation by inducing hyper-multimerization of IN and inhibiting its interaction with viral genomic RNA. The pyrrolopyridine-based ALLINI pirmitegravir (PIR) has recently advanced into phase 2a clinical trials. Previous cell culture-based viral breakthrough assays identified the HIV-1 variant that confers substantial resistance to this inhibitor.
View Article and Find Full Text PDFHIV-1 infection requires passage of the viral core through the nuclear pore of the cell, a process that depends on functions of the viral capsid. Recent studies have shown that HIV-1 cores enter the nucleus prior to capsid disassembly. Interactions of the viral capsid with the nuclear pore complex are necessary but not sufficient for nuclear entry, and the mechanism by which the viral core traverses the comparably sized nuclear pore is unknown.
View Article and Find Full Text PDFThis work reports on the extraction and characterization of the behavior of starch from residues of several potato varieties (Criolla, Sabanera and Pastusa) of Colombian origin from the Andean region using different techniques and the evaluation of the effect of citric acid (CA) on the grain morphology. Additionally, films were produced with each one of the extracted starches and glycerol. Pastusa variety starch shows a higher granule size than the other varieties and Pastusa starch shows lower amylose content compared to Sabanera and Criolla.
View Article and Find Full Text PDFAllosteric HIV-1 integrase (IN) inhibitors (ALLINIs) are investigational antiretroviral agents which potently impair virion maturation by inducing hyper-multimerization of IN and inhibiting its interaction with viral genomic RNA. The pyrrolopyridine-based ALLINI pirmitegravir (PIR) has recently advanced into Phase 2a clinical trials. Previous cell culture based viral breakthrough assays identified the HIV-1 variant that confers substantial resistance to this inhibitor.
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