Pharmacodynamics of salbutamol in humans.

Twenty patients with mild chronic bronchial asthma (females and males between 45-69 years old) treated with single dose of 0.5 mg at 1 ml of salbutamol were investigated. Measurements of heart rate, blood pressure, peak expiratory flow rate (PEFR) and plasma concentration or glucose, potassium and non-esterified fatty acids-NEFA, and salbutamol plasma levels were recorded at the same time before and 2, 5, 15, 30, 60, 75, 90, 120, 180, 240, and 360 min after salbutamol injection.

Isolation and fluorometric determination of homovanillic acid in urine and tissues.

The fluorometric method is described for determination of the final product of dopamine metabolism homovanillic acid in urine, amniotic fluid and tissue extracts. The metabolite was isolated using three chromatographic columns (1-Amberlit CG-50, 2-Dowex AG 1 x 2, 8-Aluminium oxide). Oxidation of eluates to emitter was done with potassium ferricyanide in alkalic medium.

The isolation and fluorometric determination of 3-methoxytyramine in urine and in tissues.

A fluorometric methods is described for determination of cardiac and urinary 3-methoxytyramine, a metabolite of dopamine. Methoxy metabolites were isolated on Amberlite CG-50 resin and oxidation to emitter form was performed with K3Fe(CN)6. Good specificity and sensitivity of determination, and a high recovery of 3-MT were obtained.