Purification of the protein X of Streptococcus agalactiae with a monoclonal antibody.

The protein X of Steptococcus agalactiae is a surface antigen included in the typing scheme of group B streptococci (GBS). We have developed a monoclonal antibody to the protein X and used it to purify this antigen by affinity chromatography. Electrophoresis in polyacrylamide, and immunoblotting using the monoclonal antibody or a rabbit antiserum raised with the affinity purified protein X, revealed a major band in the region of 200 kDa and a smaller one at 100 kDa.

[Allergic diagnosis of abortive chlamydial infection in the goat (author's transl)].

A delayed hypersensitivity test was used for chlamydiosis diagnosis in four goat flocks. The skin-test was performed in the neck by inoculating 100 microgram of purified Chlamydia from yolk sac or McCoy cells. The reactions were read 72 h after inoculation.