Characterization of the macrophages associated with the tunica vasculosa lentis of the rat eye.

Purpose: The tunica vasculosa lentis (TVL) is a transient vascular network surrounding the developing lens that regresses prenatally in humans and postnatally in rodents. Macrophage-like cells, sometimes referred to as hyalocytes, have been postulated to play a role in the regression of this vascular system; however, the precise identity of these cells is still unclear. The aim of the present investigation was to provide phenotypic data on these cells in combination with their three-dimensional distribution on the lens surface during the period when regression of the TVL is taking place.

Environmental scanning electron microscopic study of macrophages associated with the tunica vasculosa lentis in the developing rat eye.

Aims: To demonstrate the value of environmental scanning electron microscopy (ESEM) when used in combination with immunogold/silver enhancement methods as a valuable tool in ocular research, and to determine the phenotype of macrophages associated with the tunica vasculosa lentis while maintaining a topographical view of the lens surface.

Methods: Prenatal and postnatal rat eyes were investigated by conventional scanning electron microscopy and ESEM. In the latter case tissues were prestained with a panel of antileucocyte monoclonal antibodies and visualised with colloidal gold conjugated secondary antibody followed by silver enhancement.