Biotransformation of the Fluoroquinolone Antibiotic, Levofloxacin, by the Free and Immobilized Secretome of .

Antibiotics play a crucial role in human and animal medical healthcare, but widespread use and overuse of antibiotics poses alarming health and environmental issues. Fluoroquinolones constitute a class of antibiotics that has already become ubiquitous in the environment, and their increasing use and high persistence prompt growing concern. Here we investigated a fungal secretome prepared from the white-rot fungus , which is able to effectively degrade the environmentally persistent fluoroquinolone, levofloxacin.

A chitin-binding domain-containing gene is essential for shell development in the mollusc Tritia.

Mollusc shells are diverse in shape and size. They are created by a shell epithelium which secretes a chitinous periostracum membrane at the growing edge of the shell, and then coordinates biomineral deposition on the underside of this membrane. Although mollusc shells are important for studying the evolution of morphology, the molecular basis of the shell development is poorly understood.

From bugs to brain: unravelling the GABA signalling networks in the brain-gut-microbiome axis.

Convergent data, across species, paint a compelling picture of the critical role of the gut and its resident microbiota in several brain functions and disorders. The chemicals mediating communication along these sophisticated highways of the brain-gut-microbiome (BGM) axis include both microbiota metabolites and classical neurotransmitters. Amongst the latter, GABA is fundamental to brain function where it mediates the majority of neuronal inhibition.

Binding mechanism of oligopeptides on solid surface: assessing the significance of single-molecule approach.

This paper addresses the complementarity and potential disparities between single-molecule and ensemble-average approaches to probe the binding mechanism of oligopeptides on inorganic solids. Specifically, we explore the peptide/gold interface owing to its significance in various topics and its suitability to perform experiments both in model and real conditions. Experimental results show that the studied peptide adopts a lying configuration upon adsorption on the gold surface and interacts through its peptidic links and deprotonated thiolate extremities, in agreement with theoretical predictions.

Discovery of MDI-114215: A Potent and Selective LIMK Inhibitor To Treat Fragile X Syndrome.

LIMKs are serine/threonine and tyrosine kinases responsible for controlling cytoskeletal dynamics as key regulators of actin stability, ensuring synaptic health through normal synaptic bouton structure and function. However, LIMK1 overactivation results in abnormal dendritic synaptic development that characterizes the pathogenesis of Fragile X Syndrome (FXS). As a result, the development of LIMK inhibitors represents an emerging disease-modifying therapeutic approach for FXS.