Donors leukapheresis on Fenwal CS-3000.

Leukocytes were collected from 24 donors using Fenwal CS-3000 cell separator. Two different sedimentation agents in citric acid were used. After collection donors hematologic data were evaluated.

The influence of CS-3000 plateletapheresis on blood donors and on platelet concentrates.

Platelet concentrates were prepared from blood of donors by means of Fenwal CS-3000, or by double centrifugation method. Platelets from CS-3000 were cryopreserved with 5% DMSO for 2-4 weeks. After thawing and washing platelets were stored in room temperature for 6 hours.

Blood component therapy in open heart surgery.

Extensive bleeding still remains a major cause of morbidity and mortality in patients undergoing surgery despite the recent achievements in our understanding of hemostasis and availability of modern blood component therapy. In some surgical patients abnormally prolonged operative bleeding due to thrombocytopenia or thrombocytopathia may be corrected by the infusion of platelet concentrates. Evaluation of the requests for platelet transfusion therapy to prevent or to stop excessive thrombocytopenic bleeding should be based on clinical and laboratory data, underlying disease and availability of therapeutic products.

[Studies of the possibility of preservation of leukocyte concentrates].

The authors tried to explain whether granulocytes obtained by sedimentation on dextran can be stored, under what conditions, and for how long time. For this purpose leukocyte concentrated were stored at 4-6 degrees C and 20-24 degrees C for up to 48 hours, and the count and percent proportions of leucocytes, granulocyte viability, their phagocytic index, chemotactic ability and ATP content were determined. The investigations demonstrated a progressing decrease of the biological viability of granulocytes after 24 hours, and a further decrease after 48 hours.