End-Product Control of Carbon Metabolism in Culture-Grown Sugar Beet Plants (Molecular and Physiological Evidence on Accelerated Leaf Development and Enhanced Gene Expression).

Sugar beet (Beta vulgaris L.) seedlings were grown on media containing 90 to 300 mM sucrose or glucose. Compared to controls, sugar-grown plants had higher growth rate, photosynthesis, and leaf sugar levels.

Cytosolic fructose-1,6-bisphosphatase: A key enzyme in the sucrose biosynthetic pathway.

Fructose-1,6 bisphosphatase (FBPase) is a ubiquitous enzyme controlling a key reaction. In non-photosynthetic tissues, it regulates the rate of gluconeogenesis. In photosynthetic tissues, two FBPase isozymes (chloroplastic and cytosolic) play key roles in carbon assimilation and metabolism.

Purification and Light-Dependent Molecular Modulation of the Cytosolic Fructose-1,6-Bisphosphatase in Sugarbeet Leaves.

Cytosolic fructose-1,6-bisphosphatase (FBPase) was purified 472-fold from sugarbeet (Beta vulgaris L.) leaves by ammonium sulfate fractionation, anion-exchange chromatography (DEAE Sepharose), cation-exchange chromatography (S-Sepharose), gel filtration (Sephacryl S-300), and hydrophobic interaction chromatography (Phenyl Sepharose). The dissociated polypeptide (molecular mass of 37 kD) was used to generate polyclonal antibodies.