Publications by authors named "J D Schulzke"
Article Synopsis
- Traditional cell line monolayers have been the standard for studying epithelial barrier and transport, but 3D intestinal organoids are emerging as a better model for studying these functions.
- Researchers developed human duodenum-derived organoid monolayers that don't require a gelatinous matrix for anchorage, which could hinder diffusion.
- The new organoid monolayers effectively replicate the duodenum’s functions and composition, showing improved models for studying transport mechanisms without relying on animal-derived materials.
Introduction: Human infections with the food-borne enteropathogen are responsible for increasing incidences of acute campylobacteriosis cases worldwide. Since antibiotic treatment is usually not indicated and the severity of the enteritis directly correlates with the risk of developing serious autoimmune disease later-on, novel antibiotics-independent intervention strategies with non-toxic compounds to ameliorate and even prevent campylobacteriosis are utmost wanted. Given its known pleiotropic health-promoting properties, curcumin constitutes such a promising candidate molecule.
View Article and Find Full Text PDFBackground: In patients with diarrhea-predominant irritable bowel syndrome (IBS-D), the diarrheal mechanisms are largely unknown, and they were examined in this study on colon biopsies.
Methods: Electrophysiological measurements were used for monitoring functional changes in the diarrheic colon specimens. In parallel, tight junction protein expression was analyzed by Western blot and confocal laser-scanning microscopy, and signaling pathway analysis was performed using RNA sequencing and bioinformatics.
The oleoresin myrrh has been used for centuries as an anti-inflammatory remedy for a variety of diseases and is said to have a protective effect on the intestinal epithelium. An intact epithelial barrier function is the prerequisite for a healthy gut. Inflammatory and infectious diseases of the intestine, in particular, lead to barrier impairment resulting in leak-flux diarrhea and mucosal immune responses.
View Article and Find Full Text PDFBackground: toxins TcdA and TcdB are responsible for diarrhea and colitis. Lack of functional studies in organoid models of the gut prompted us to elucidate the toxin's effects on epithelial barrier function and the molecular mechanisms for diarrhea and inflammation.
Methods: Human adult colon organoids were cultured on membrane inserts.