Activation by TyrR in Escherichia coli K-12 by Interaction between TyrR and the α-Subunit of RNA Polymerase.

A novel selection was developed for mutants of the C-terminal domain of RpoA (α-CTD) altered in activation by the TyrR regulatory protein of Escherichia coli K-12. This allowed the identification of an aspartate to asparagine substitution at residue 250 (DN250) as an activation-defective (Act) mutation. Amino acid residues known to be close to D250 were altered by mutagenesis, and the substitutions DR250, RE310, and RD310 were all shown to be defective in activation.

Eta Carinae's Thermal X-ray Tail Measured with and .

The evolved, massive highly eccentric binary system, Car, underwent a periastron passage in the summer of 2014. We obtained two coordinated X-ray observations with and during the elevated X-ray flux state and just before the X-ray minimum flux state around this passage. These observations clearly detected X-ray emission associated with Car extending up to ~50 keV for the first time.

Self blood glucose monitoring in type 2 diabetes. A financial impact analysis based on UK primary care.

Background: UK consensus guidelines recommend limited use of self-monitoring of blood glucose (SMBG) in patients with type 2 diabetes using diet and exercise, metformin and/or a glitazone. This analysis quantifies the usage of and costs associated with SMBG in type 2 diabetes according to treatment regimen.

Methods: Prevalence data for diabetes were assessed using UK Quality and Outcomes Framework returns for 2006/2007.

Biosynthesis of the Aromatic Amino Acids.

This chapter describes in detail the genes and proteins of Escherichia coli involved in the biosynthesis and transport of the three aromatic amino acids tyrosine, phenylalanine, and tryptophan. It provides a historical perspective on the elaboration of the various reactions of the common pathway converting erythrose-4-phosphate and phosphoenolpyruvate to chorismate and those of the three terminal pathways converting chorismate to phenylalanine, tyrosine, and tryptophan. The regulation of key reactions by feedback inhibition, attenuation, repression, and activation are also discussed.