Publications by authors named "J D Hardcastle"

Article Synopsis
  • DNA methylation is a key epigenetic change in humans, used as a biomarker in diagnosing various diseases, but current methods overlook age and sex-specific patterns.
  • The study analyzed DNA methylation from blood samples of healthy individuals and patients with specific syndromes, introducing a Generalized Additive Model to assess 700,000 CpG sites while accounting for age and sex differences.
  • The research achieved a high prediction accuracy of 0.96 in identifying abnormal methylation patterns, demonstrating the effectiveness of their custom machine learning pipeline for diagnosing potential congenital disorders.
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The COVID-19 pandemic has revealed the importance of the detection of airborne pathogens. Here, we present composite air filters featuring a bioinspired liquid coating that facilitates the removal of captured aerosolized bacteria and viruses for further analysis. We tested three types of air filters: commercial polytetrafluoroethylene (PTFE), which is well known for creating stable liquid coatings, commercial high-efficiency particulate air (HEPA) filters, which are widely used, and in-house-manufactured cellulose nanofiber mats (CNFMs), which are made from sustainable materials.

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Antifouling membranes that offer excellent operational lifetimes are critical technologies needed to meet the growing demand for clean water. In this study, we demonstrate antifouling membranes featuring an ultrathin oil layer that stayed immobilized on the surface and in the pore walls of poly(vinylidene fluoride) membranes for multiple cycles of operation at industrially relevant transmembrane pressures. An optimized quantity of a commercial Krytox oil with either a low (K103) or a high viscosity (K107) was infused onto the active surface and into the pores of membranes with a 0.

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In response to calls for curricular materials that integrate molecular genetics and evolution and adhere to the K-12  (NGSS), the Genetic Science Learning Center (GSLC) at the University of Utah has developed and tested the "Evolution: DNA and the Unity of Life" curricular unit for high school biology. The free, 8-week unit illuminates the underlying role of molecular genetics in evolution while providing scaffolded opportunities to engage in making arguments from evidence and analyzing and interpreting data.  We used a randomized controlled trial design to compare student learning when using the new unit with a condition in which teachers used their typical (NGSS-friendly) units with no molecular genetics.

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