Publications by authors named "J Claire"

Background: Creeping indigo (Indigofera spicata) toxicosis is an emerging problem among horses in Florida and bordering states.

Objectives: To quantify the putative toxins l-indospicine (IND) and 3-nitropropionic acid (NPA) in creeping indigo collected from multiple sites and to measure plasma toxin concentrations in ponies fed creeping indigo and horses with presumptive creeping indigo toxicosis.

Study Design: Experimental descriptive study with descriptive observational field investigation.

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Background: Measurements of water consumed by fed healthy horses might not apply to horses that are unwilling or unable to drink or are not fed for any reason.

Objective: To examine the effects of feed deprivation on voluntary water consumption compared with fed conditions.

Study Design: In vivo experiment.

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Article Synopsis
  • A fluorometric assay was developed to quantify co-purified prymnesins-1 and -2 from Prymnesium parvum cultures, revealing higher concentrations of these exotoxins in the culture medium compared to the cell's endotoxins.
  • Throughout the growth cycle, exotoxin levels remained low until post-log growth, where they surged, while endotoxin levels peaked during late- and post-log phases before declining.
  • Cultures varied in exotoxin production based on salinity and other conditions; for example, higher salinity (30 PSU) resulted in lower exotoxin levels, and phosphate limitation led to increased exotoxin concentrations per cell, particularly in later growth phases.
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Increasingly over the past century, seasonal fish kills associated with toxic blooms of Prymnesium parvum have devastated aquaculture and native fish, shellfish, and mollusk populations worldwide. Protracted blooms of P. parvum can result in major disturbances to the local ecology and extensive monetary losses.

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