Publications by authors named "J Ceusters"
Revealing the Therapeutic Potential of Muscle-Derived Mesenchymal Stem/Stromal Cells: An In Vitro Model for Equine Laminitis Based on Activated Neutrophils, Anoxia-Reoxygenation, and Myeloperoxidase.
Animals (Basel)
September 2024
Article Synopsis
- - Laminitis in horses causes severe pain and can lead to euthanasia, prompting research for better treatments through an in vitro model that tests cell responses to stressful conditions.
- - The study found that during reoxygenation, keratinocytes (skin cells) show reduced metabolism, indicating cellular stress, but introducing muscle-derived stem cells helped restore their function.
- - Additionally, exposure to activated neutrophils increased cell activity related to inflammation, which stem cells could help regulate, suggesting their potential use in treating laminitis through cell therapy.
Article Synopsis
- - The study explores the use of equine platelet lysate (ePL) as a replacement for fetal bovine serum (FBS) in growing muscle-derived mesenchymal stromal cells (mdMSCs) for regenerative medicine, addressing ethical and practical issues associated with FBS.
- - Two innovative 2D and 3D culture models were tested, with the 3D model yielding a higher total cell count than the 2D model, while both models confirmed the multipotent capabilities of mdMSCs through differentiation assays.
- - The mdMSCs showed promising immunomodulatory properties, significantly inhibiting myeloperoxidase (MPO) and reactive oxygen species (ROS) activity, especially in the
Crassulacean acid metabolism (CAM) leaves are characterized by nocturnal acidification and diurnal deacidification processes related with the timed actions of phosphoenolpyruvate carboxylase and Rubisco, respectively. How CAM leaves manage cytosolic proton homeostasis, particularly when facing massive diurnal proton effluxes from the vacuole, remains unclear. A 12-phase flux balance analysis (FBA) model was constructed for a mature malic enzyme-type CAM mesophyll cell in order to predict diel kinetics of intracellular proton fluxes.
View Article and Find Full Text PDFThe main causes of mortality in horses are the gastrointestinal pathologies associated with septic shock. Stem cells have shown, through systemic injection, a capacity to decrease inflammation and to regenerate injured tissue faster. Nevertheless, to achieve this rapid and total regeneration, systemic injections of 1 to 2 million cells per kilogram of body weight must be considered.
View Article and Find Full Text PDFImmune checkpoint inhibitor (ICI) therapy has proven revolutionary in the treatment of some cancers. However, ovarian cancer remains unresponsive to current leading ICIs, such as anti-PD1 or anti-PD-L1. In this article, we explored the potential of an upcoming checkpoint molecule, T-cell immunoglobulin and mucin domain 3 (TIM3), for the treatment of ovarian cancer using a syngeneic orthotopic mouse model (ID8-fLuc).
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