PCR/restriction fragment length polymorphism (RFLP) typing of human and poultry Campylobacter jejuni strains.

Aims: The PCR/RFLP typing of 156 isolates Campylobacter jejuni originating from poultry and humans was performed (101 human and 55 poultry strains).

Methods And Results: On the basis of restrictive digest, six types were identified with AfaI, seven types with MboI and five types with HaeIII. With a combination of these three enzymes, 22 types were found.

Identification of thermophilic Campylobacter spp. by phenotypic and molecular methods.

Aims: The differences between phenotyping and genotyping (polymerase chain reaction- restriction fragment length polymorphism) of Campylobacter jejuni, Campylobacter coli, Campylobacter lari and Campylobacter upsaliensis were assessed.

Methods And Results: A total of 51, 63 and 88 strains from dogs, pigs and humans, respectively, were examined. The strains were first typed by biochemical methods, then by PCR-RFLP using AluI and Tsp509I.