Stereospecific analysis of fatty acid esters of chloropropanediol isolated from fresh goat milk.

The fatty acid esters of chloropropanediol isolated from goat milk fat in small quantities were subjected to a stereospecific analysis via phospholipase C and phosphocholine esters as intermediates. Synthetic rac-1-chloro-2,3-dioleoyl-propanediol was prepared by standard methods and was used as a control. The stereospecific analyses were performed following a release of the fatty acids from the primary positions of each chloropropanediol diester with pancreatic lipase.

Comparative study of the molecular species of chloropropanediol diesters and triacylglycerols in milk fat.

In an effort to establish the origin of the fatty acid esters of 3-chloropropanediol, which recently have been isolated in small amounts from goat milk, we compared the molecular species composition of the chlorohydrin diesters and of goat milk triacylglycerols. The chloropropanediol diesters were found to be composed of molecular species containing C10-C18 fatty acids and corresponded closely in carbon number to those calculated for the long chain sn-1,2-diacylglycerol moieties of goat milk triacylglycerols. The molecular species of goat milk total triacylglycerols contained C4-C18 fatty acids.

Composition of the hydrocarbon fraction of goats' milk.

The hydrocarbon fraction of the neutral lipids of goats' milk was chromatographically purified and analyzed by gas-liquid chromatography and mass spectrometry. The goats' milk samples, which were collected during the spring of the year, represent a cross-sectional analysis; the purified hydrocarbon fraction displays a broad spectrum of compounds. The major components of the hydrocarbon fraction identified for the first time in goats' milk were 3,7,11,15-tetramethylhexadec-2-ene (phytene-2) (1.

Fatty acid composition of polar lipids in goats' milk.

Silicic acid column chromatography was used to separate the polar lipids of goats' milk into glycolipid, phosphatidylethanolamine, phosphatidylserine plus phosphatidylinositol, phosphatidylcholine, and sphingomyelin fractions. Each fraction was purified by column chromatography and its fatty acid profile determined by gas liquid chromatography and mass spectrometry. The glycerophospholipids each contained 18:1 as the predominant fatty acid (approximately 45%).

Composition of the milks of dairy cattle. II. Ash, calcium, magnesium, and phosphorus.

Milks from commercial dairy herds in Southeastern Pennsylvania were analyzed for ash, calcium, magnesium, and phosphorus. Milk samples were from single milkings of 151 healthy purebred cows in midlactation. The data represent market milk composition.