Reproduction of attenuated mumps virus in dog kidney cell cultures precultivated at 37 and 34 degrees C.

The titres of attenuated mumps virus reached in primary dog kidney cell cultures precultivated at 34 degrees C were on the average 8-fold higher than in cultures precultivated at 37 degrees C. This effect was probably due to adaptation of the cells to lower temperature, connected with changes in cell metabolism, which were also manifested by different growth and survival of the cell cultures on glasses of different composition.

Reproduction of mumps virus strains in Vero cell cultures at 32, 37 and 40 degrees C.

Reproduction of various mumps virus strains was studied in Vero cell tube cultures at 32, 37 and 40 degrees C. Strains of different character could be distinguished based on temperature dependence of their reproduction, but results of several repeated experiments had to be taken into account. The problem of a suitable numerical marker was discussed along with the relationship between temperature dependence of reproduction of a strain and the host response to its action in vivo.