Publications by authors named "J Calvo-Montes"
Herein, we combined different bioinformatics tools and databases (BV-BRC, ResFinder, RAST, and KmerResistance) to perform a prediction of antimicrobial resistance (AMR) in the genomic sequences of 107 Corynebacterium striatum isolates for which trustable antimicrobial susceptibility (AST) phenotypes could be retrieved. Then, the reliabilities of the AMR predictions were evaluated by different metrics: area under the ROC curve (AUC); Major Error Rates (MERs) and Very Major Error Rates (VMERs); Matthews Correlation Coefficient (MCC); F1-Score; and Accuracy. Out of 15 genes that were reliably detected in the C.
View Article and Find Full Text PDFWithin-host transition to GES-55 during a GES-6-producing Serratia marcescens outbreak: Emergence of ceftazidime-avibactam resistance and increased susceptibility to carbapenems.
Int J Antimicrob Agents
August 2024
Article Synopsis
- The study tracks the emergence of resistance to ceftazidime-avibactam in a type of antibiotic-resistant bacteria (GES-type carbapenemases) during an unusual outbreak of Serratia marcescens in Spain during COVID-19.* ! -
- Researchers conducted a retrospective analysis using whole genome sequencing to understand the relationships between bacterial samples from patients and their environment, discovering a genetic connection and plasmid transfer related to resistance.* ! -
- Key findings include the appearance of a mutation that facilitated the development of resistance to ceftazidime-avibactam, highlighting the urgent need for improved diagnostic tools and better antimicrobial usage strategies to combat such outbreaks.* !
Identification of hypermucoviscous Klebsiella pneumoniae K1, K2, K54 and K57 capsular serotypes by Raman spectroscopy.
Spectrochim Acta A Mol Biomol Spectrosc
October 2024
Antimicrobial resistance poses a significant challenge in modern medicine, affecting public health. Klebsiella pneumoniae infections compound this issue due to their broad range of infections and the emergence of multiple antibiotic resistance mechanisms. Efficient detection of its capsular serotypes is crucial for immediate patient treatment, epidemiological tracking and outbreak containment.
View Article and Find Full Text PDFEpidemiological and clinical characterization of community, healthcare-associated and nosocomial colonization and infection due to carbapenemase-producing Klebsiella pneumoniae and Escherichia coli in Spain.
Infection
December 2024
Article Synopsis
- Community-acquired (CA) and healthcare-associated (HCA) infections caused by carbapenemase-producing Enterobacterales (CPE), particularly Klebsiella pneumoniae (CP-Kp) and Escherichia coli (CP-Ec), are not well understood, prompting a study of their clinical and molecular characteristics.
- A cohort study involving 59 Spanish hospitals analyzed 386 patients, finding that 76.3% had infections caused by CPE, with most cases being HCA or nosocomial, and urinary tract infections as the most common infection type.
- The study revealed a significant mortality rate of 28% for those with infections, with nosocomial infections having higher mortality rates; understanding these patterns
Gene sequencing in back of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the current approach for discriminating infections produced by different severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in the clinic. However, sequencing is often a time-consuming step, which hinders the deployment of a very fast response during a pandemic. Here, we propose to run a CRISPR-Cas12a reaction after completing the RT-qPCR and in the very same pot to detect with high specificity genetic marks characterizing variants of concern.
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