Publications by authors named "J Cacho-Calvo"
Challenges Facing Two Outbreaks of Carbapenem-Resistant : From Cefiderocol Susceptibility Testing to the Emergence of Cefiderocol-Resistant Mutants.
Antibiotics (Basel)
August 2024
Carbapenem-resistant (CRAB) infections are associated with poor outcomes depending on patient's conditions, clinical severity and type of infection, and treatment is challenging given the limited therapeutic options available. The aim of this study was to describe the clinical and microbiological characteristics of two outbreaks caused by CRAB in an intensive care unit (ICU). In addition, the mechanisms of resistance detected in these strains and the treatment chosen according to the available therapeutic options were analyzed.
View Article and Find Full Text PDFUtility of culture and molecular methods using Allplex Bacterial Vaginosis Plus Assay (Seegene) as a tool for endometriosis, infertility and recurrent pregnancy loss diagnosis.
Diagn Microbiol Infect Dis
November 2024
Endometriosis, infertility, or recurrent pregnancy loss (RPL) are entities characterised by a decrease in Lactobacillus spp. and an increase in bacterial vaginosis-associated bacteria, (BVAV) according with 16S rRNA sequencing studies. However, the use of nucleic acid amplification tests (NAAT) as a tool for diagnosis algorithms is unknown.
View Article and Find Full Text PDFArticle Synopsis
- - An increase in gonococcal infections has been observed in Europe and Spain, leading to more cases of disseminated gonococcal infection, which can include gonococcal arthritis.
- - Five cases of gonococcal arthritis were reported in a Madrid hospital between October 2022 and October 2023, primarily affecting male patients with a history of unprotected sex.
- - Advances in molecular techniques have improved the identification of culture-negative gonococcal arthritis cases and have revealed mutations linked to fluoroquinolone resistance, prompting a shift towards oral treatments.
Comparison of gradient diffusion and molecular methods using Allplex™ NG&DR assay (Seegene®) for macrolide and fluoroquinolone screening resistance in Neisseria gonorrhoeae.
Eur J Clin Microbiol Infect Dis
May 2024
Article Synopsis
- * The study compared a gradient diffusion test to a new molecular method (Allplex™ NG&DR assay) for detecting mutations linked to resistance in 100 NG isolates, finding no significant correlation for fluoroquinolone resistance but identified a high rate of S91F mutations.
- * While the molecular method showed good sensitivity (96.1%) and specificity (90.9%) for fluoroquinolone resistance, it cannot replace the gradient diffusion test for macrolides due to lack of positive results for resistance mutations.