Understanding TCR T cell knockout behavior using interpretable machine learning.

Genetic perturbation of T cell receptor (TCR) T cells is a promising method to unlock better TCR T cell performance to create more powerful cancer immunotherapies, but understanding the changes to T cell behavior induced by genetic perturbations remains a challenge. Prior studies have evaluated the effect of different genetic modifications with cytokine production and metabolic activity assays. Live-cell imaging is an inexpensive and robust approach to capture TCR T cell responses to cancer.

Central control of dynamic gene circuits governs T cell rest and activation.

The ability of cells to maintain distinct identities and respond to transient environmental signals requires tightly controlled regulation of gene networks. These dynamic regulatory circuits that respond to extracellular cues in primary human cells remain poorly defined. The need for context-dependent regulation is prominent in T cells, where distinct lineages must respond to diverse signals to mount effective immune responses and maintain homeostasis.

Development and Implementation of a Basic Veterinary Ultrasound Curriculum.

Diagnostic ultrasound is an important imaging modality in veterinary medicine. Surveys of veterinarians suggest that ultrasound is a desired clinical competence and that new graduates are expected to practice basic ultrasound skills. This report describes the development and implementation of a basic ultrasound training program in the core curriculum at Iowa State University College of Veterinary Medicine (ISU-CVM).