Conserved ancillary residues situated proximally to the VIM-2 active-site affect its metallo β-lactamase activity.

Verona-integron-metallo-β-lactamase (VIM-2) is one of the most widespread class B β-lactamase responsible for β-lactam resistance. Although active-site residues help in metal binding, the residues nearing the active-site possess functional importance. Here, to decipher the role of such residues in the activity and stability of VIM-2, the residues E146, D182, N210, S207, and D213 were selected through in-silico analyses and substituted with alanine using site-directed mutagenesis.

Work-related musculoskeletal disorders among various occupational workers in India: a systematic review and meta-analysis.

Objectives: Work-related musculoskeletal disorders (WMSDs) are among the most common occupational diseases, affecting various sectors such as agriculture, small-scale industries, handicrafts, construction, and banking. These disorders, caused by overexertion and repetitive motion, lead to work absenteeism, productivity loss, and economic impacts. The aim of the study was to determine the magnitude of musculoskeletal disorders among different occupational workers in India.

Analysis of the interaction of influenza a virus nucleoprotein with host cell nucleolin.

Targeting interactions between a virus and a host protein is one of the important approaches to developing antiviral therapies. We previously identified host nucleolin as a novel interacting partner of the influenza A virus nucleoprotein, and it was demonstrated that this interaction restricts virus replication. In the current study, we examined the interaction of nucleolin with the viral nucleoprotein at the domain and amino acid levels using in vitro and in silico approaches.