High Aspect Ratio Polymer Nanocarriers for Gene Delivery and Expression in Plants.

Plant genetic engineering methods are critical for food security and biofuel production and to enable molecular farming. Here, we elucidated how polymeric high aspect ratio nanocarriers can enable DNA delivery to plants and transient expression. We demonstrated that a nanocarrier with 20 nm width, 80 nm length, and a polymer-to-DNA ratio of N/P = 3.

Exploring Oxidative Stress and Metabolic Dysregulation in Lung Tissues of Offspring Rats Exposed to Prenatal Polystyrene Microplastics: Effects of Melatonin Treatment.

Metabolomics research provides a clearer understanding of an organism's metabolic state and enables a more accurate representation of its functional performance. This study aimed to investigate changes in the metabolome of lung tissues resulting from prenatal exposure to polystyrene microplastics (PS-MPs) and to understand the underlying mechanisms of lung damage in rat offspring. We conducted metabolomic analyses of lung tissue from seven-day-old rat pups exposed to prenatal PS-MPs.

Personalized, autologous neoantigen-specific T cell therapy in metastatic melanoma: a phase 1 trial.

New treatment approaches are warranted for patients with advanced melanoma refractory to immune checkpoint blockade (ICB) or BRAF-targeted therapy. We designed BNT221, a personalized, neoantigen-specific autologous T cell product derived from peripheral blood, and tested this in a 3 + 3 dose-finding study with two dose levels (DLs) in patients with locally advanced or metastatic melanoma, disease progression after ICB, measurable disease (Response Evaluation Criteria in Solid Tumors version 1.1) and, where appropriate, BRAF-targeted therapy.

ABA-activated low-nanomolar Ca-CPK signalling controls root cap cycle plasticity and stress adaptation.

Abscisic acid (ABA) regulates plant stress adaptation, growth and reproduction. Despite extensive ABA-Ca signalling links, imaging ABA-induced increases in Ca concentration has been challenging, except in guard cells. Here we visualize ABA-triggered [Ca] dynamics in diverse organs and cell types of Arabidopsis thaliana using a genetically encoded Ca ratiometric sensor with a low-nanomolar Ca-binding affinity and a large dynamic range.

Clinical presentation, management and outcome of maxillofacial fractures in pet rabbits: 27 cases (2008-2022).

Objectives: To report clinical findings, management strategies and outcomes in pet rabbits with maxillofacial fractures.

Materials And Methods: Medical records of pet rabbits with confirmed maxillofacial fractures from three exotic animal veterinary services between 2008 and 2022 were reviewed.

Results: Forty-five fractured maxillofacial bones were reported in 27 rabbits, including mandibular symphyseal separation in 13 rabbits.