Devices and techniques used to obtain and analyze three-dimensional cell cultures.

Cell cultures are indispensable for both basic and applied research. Advancements in cell culture and analysis increase their utility for basic research and translational applications. A marked development in this direction is advent of three-dimensional (3D) cultures.

Leptospirosis in Reunion Island (Indian Ocean): analysis of factors associated with severity in 147 confirmed cases.

Objective: Analysis of risk factors associated with severity in patients with confirmed leptospirosis.

Design And Setting: Retrospective study in 147 leptospirosis-confirmed patients at two tertiary nonteaching hospital in Reunion Island.

Patients: 138 men and 9 women, aged 36+/-14 years, 80 in the ICU and 67 in medical wards.

Lack of arrhythmogenicity of aminophylline in dogs.

This study was designed to determine if doses of aminophylline up to 10 mg/kg given i.v. would produce ventricular arrhythmias in seven healthy dogs anaesthetized with fentanyl-droperidol-pentobarbital.

Small-volume resuscitation with hypertonic saline solution in hypovolemic cats.

We evaluated the hemodynamic effects of IV and intraaortic (aortic root) administration of 7.5% NaCl solution on hemodynamics in anesthetized cats with severe hypovolemia. Hypovolemic shock was induced by exsanguinating cats to a mean arterial blood pressure of 50 mm of Hg, which was maintained for 30 minutes prior to treatment.

Cardiorespiratory effects of the intravenous administration of tiletamine-zolazepam to dogs.

The anesthetic, hemodynamic, and respiratory effects of an intravenously administered 1:1 combination of tiletamine and zolazepam were evaluated in dogs. Each dog received tiletamine-zolazepam (6.6, 13.