Transcription factor exchange enables prolonged transcriptional bursts.

Single-molecule imaging inside living cells has revealed that transcription factors (TFs) bind to DNA transiently, but a long-standing question is how this transient binding is related to transcription activation. Here, we devised a microscopy method to simultaneously measure transient TF binding at a single locus and the effect of these binding events on transcription. We show that DNA binding of the yeast TF Gal4 activates transcription of a target gene within a few seconds, with at least ∼20% efficiency and with a high initiation rate of ∼1 RNA/s.

Time will tell: comparing timescales to gain insight into transcriptional bursting.

Recent imaging studies have captured the dynamics of regulatory events of transcription inside living cells. These events include transcription factor (TF) DNA binding, chromatin remodeling and modification, enhancer-promoter (E-P) proximity, cluster formation, and preinitiation complex (PIC) assembly. Together, these molecular events culminate in stochastic bursts of RNA synthesis, but their kinetic relationship remains largely unclear.

Evaluating the impact of drying on leaching from a solidified/stabilized waste using a monolithic diffusion model.

The release rates of constituents of potential concern from solidified/stabilized cementitious waste forms are potentially impacted by drying, which, however, is not well understood. This study aimed to identify the impacts of drying on subsequent leaching from Cast Stone as an example of a solidified cementitious waste form. The release fluxes of constituents from monoliths after aging under 100, 68, 40, and 15 % relative humidity for 16, 32, and 48 weeks, respectively, were derived from mass transfer tank leaching tests following EPA Method 1315.

Transcription factor clusters enable target search but do not contribute to target gene activation.

Many transcription factors (TFs) localize in nuclear clusters of locally increased concentrations, but how TF clustering is regulated and how it influences gene expression is not well understood. Here, we use quantitative microscopy in living cells to study the regulation and function of clustering of the budding yeast TF Gal4 in its endogenous context. Our results show that Gal4 forms clusters that overlap with the GAL loci.

Impact of oxidation and carbonation on the release rates of iodine, selenium, technetium, and nitrogen from a cementitious waste form.

Evaluation of the long-term retention mechanisms and potential release rates for the primary constituents of potential concern (COPCs) (i.e., Tc, I, Se, and nitrate) is necessary to determine if Cast Stone, a radioactive waste form, can meet performance objectives under near-surface disposal scenarios.