Injectable thermosensitive antibiotic-laden chitosan hydrogel for regenerative endodontics.

Injectable biomaterials, such as thermosensitive chitosan (CH)-based hydrogels, present a highly translational potential in dentistry due to their minimally invasive application, adaptability to irregular defects/shapes, and ability to carry therapeutic drugs. This work explores the incorporation of azithromycin (AZI) into thermosensitive CH hydrogels for use as an intracanal medication in regenerative endodontic procedures (REPs). The morphological and chemical characteristics of the hydrogel were assessed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR).

High-Frequency Ultrasound for Detecting Periodontal Inflammation: A Preclinical Diagnostic Accuracy Study.

Aim: Ultrasonography (US) has shown accuracy in imaging healthy periodontium. This study aims to evaluate the feasibility and accuracy of US for estimating dimensions of inflamed periodontium induced by ligature and bacteria.

Methods: Periodontal tissues of maxillary as well as mandibular premolars and molars in six female mini pigs were treated with ligature and three strains of bacteria for 4-10 weeks.

Ultrasonographic Evaluation of Vascular Response to Mechanical Compression during Induced Gingival Inflammation.

The aim of this study was to evaluate the gingival vascular response to mechanical compression during inflammation using ultrasonography. Four female and 4 male Sinclair mini pigs 18 mo of age were included in the study. Pathogenic bacteria-impregnated silk ligatures were placed around the third premolars (PM3), fourth premolars (PM4), and first molars (M1).

Functional characterization and optimization of protein expression in shuttle plasmids.

Unlabelled: Oral spirochetes are among a small group of keystone pathogens contributing to dysregulation of periodontal tissue homeostasis, leading to breakdown of the tissue and bone supporting the teeth in periodontal disease. Of the greater than sixty oral species and phylotypes, is one of the few that can be grown in culture and the only one in which genetic manipulation has been shown to be practicable. is thus a model organism for studying spirochete metabolic processes, interactions with other microbes and host cell and tissue responses relevant to oral diseases as well as venereal and nonvenereal treponematoses.