Publications by authors named "J Bomberger"
Article Synopsis
- Reproducibility is crucial in science as it boosts confidence in findings and enables comparison of data, yet evaluating it can be challenging, especially with RNA sequencing (RNA-seq) where multiple steps can introduce variance.
- This study specifically examines the reproducibility of gene expression data from bacteria in cystic fibrosis models, utilizing samples from three labs and different sequencing pipelines to draw comparisons.
- The results indicate high reproducibility of gene expression across labs, despite some variance introduced by different sequencing methods, with both pipelines detecting over 80% of the same differentially expressed genes, confirming the validity of RNA-seq data comparisons.
Laboratory models are central to microbiology research, advancing the understanding of bacterial physiology by mimicking natural environments, from soil to the human microbiome. When studying host-bacteria interactions, animal models enable investigators to examine bacterial dynamics associated with a host, and in the case of human infections, animal models are necessary to translate basic research into clinical treatments. Efforts toward improving animal infection models are typically based on reproducing host genotypes/phenotypes and disease manifestations, leaving a gap in how well the physiology of microbes reflects their behavior in a human host.
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- Elexacaftor/tezacaftor/ivacaftor (ETI) therapy has significantly improved cystic fibrosis (CF) treatment, yet its impact on sinus microbiota remains unknown.
- A study involving 38 adults with CF and chronic rhinosinusitis examined sinus samples pre- and post-ETI using advanced sequencing methods.
- Results showed that while total bacterial load and diversity didn't significantly change after ETI, certain bacterial species, including methicillin-resistant Staphylococcus aureus (MRSA), persisted, indicating the need for ongoing management of these pathogens in CF care.
Mucosa-associated biofilms are associated with many human disease states, but the host mechanisms promoting biofilm remain unclear. In chronic respiratory diseases like cystic fibrosis (CF), Pseudomonas aeruginosa establishes chronic infection through biofilm formation. P.
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