Novel, fast, and reliable electrochemical dsDNA biosensor based on O-terminated pristine nanocrystalline boron-doped diamond electrode for DNA interaction studies.

We present a novel application of a nanocrystalline boron-doped diamond electrode (B-NCDE) for the construction of an electrochemical DNA biosensor based on double-stranded DNA (dsDNA) for various bioanalytical applications. Surface characterization of the transducer surface (prior and after the fabrication of negatively charged O-terminated surface - O-B-NCDE) was performed by scanning electron microscopy (SEM), Raman spectroscopy, and linear sweep voltammetry (LSV) that was further used for the voltammetric determination, scan rate dependence investigation, and repeatability examination of dsDNA electrochemical oxidation at the O-B-NCDE. The fabrication of a dsDNA/O-B-NCDE biosensor via electrostatic adsorption of dsDNA involved a thorough optimization process of deposition potential (E), deposition time (t), and optimal saturation concentration (c) with optimal values of 0.

A novel impedimetric sensor based on N-acetyl-L-cysteine for the determination of hydroxyl radicals in cell cultures in vitro.

We report a novel impedimetric sensor based on a graphite electrode impregnated with polyethylene and paraffin under vacuum (IGE) modified with electrochemically deposited gold and a self-assembled monolayer of N-acetyl-L-cysteine (NAC/Au/IGE) for selective and sensitive determination of extracellular hydroxyl radicals (OH) generated by living cells. The application of a sulphur-containing molecule oxidized by OH predicts the high selectivity of the sensor, and the utilization of the non-faradaic impedance spectroscopy for recording an analytical response makes it possible to achieve superior sensitivity with a detection limit of 0.01 nM and a linear dynamic range of 0.

Adsorptive stripping voltammetric determination of carbofuran in food using novel type of modified carbon-based electrode with grafted layers of nickel.

A voltammetric determination of carbofuran (CBF) was developed using a novel type of carbon-containing electrode (CCE) modified with carbon ink (CI) and a chromatographic sorbent (CS) based of chromaton (Ch), polyethylene glycol and nickel acetylacetonate with grafted layers of nickel (NiCS, stands for Ni modified CS) further denoted as CI/NiCS/CCE. The surface morphology of this modified electrode was investigated by scanning electron microscopy (SEM) and by electrochemical impedance spectroscopy (EIS). CBF which is not electrochemically oxidizable was first hydrolyzed in alkaline medium to give anodically active phenolic analogue CBFP.

Graphene derivatives-based electrodes for the electrochemical determination of carbamate pesticides in food products: A review.

Graphene (GR) composites have great potential for the determination of carbamates pesticides (CPs) by electrochemical methods. Since the beginning of the 20th century, GR has shown remarkable promise as electrode material for various sensors. The contamination of food products with harmful CPs is a major problem as they do not always damage human health immediately, but can be harmful after prolonged exposure.

Voltammetric studies of the interaction of genotoxic 2-nitrofluorene with DNA.

The interaction of genotoxic environmental pollutant 2-nitrofluorene (2-NF) with double-stranded DNA has been studied using a hanging mercury drop electrode (HMDE) as an electrochemical sensor. Two types of DNA damage were investigated and electrochemically detected using cyclic voltammetry and differential pulse voltammetry: (i) DNA damage caused by the direct interaction with 2-NF and (ii) DNA damage caused by short-lived radicals generated by the electrochemical reduction of 2-NF. For the study of the direct interaction, the HMDE was modified by DNA and the interaction of DNA with 2-NF was studied after their mutual interaction right at the HMDE surface, or DNA was preincubated with 2-NF in solution and, subsequently, the interaction was studied voltammetrically.