Purinergic control of apical ion conductance by luminal ATP in rat colonic epithelium.

ATP, released e.g. after cell damage or during inflammation, can alter ion transport across the intestinal mucosa via stimulation of purinergic receptors in the basolateral as well as in the apical membrane of epithelial cells.

Time-dependent effects of tumor necrosis factor α on Ca-dependent secretion in murine small intestinal organoids.

Background: Intestinal organoids are stem cell-derived, 3D "mini-guts" with similar functions as the native intestinal epithelium such as electrolyte transport or establishment of an epithelial barrier. During intestinal inflammation, epithelial functions are dysregulated by proinflammatory cytokines like tumor necrosis factor α (TNFα) and other messengers from the immune system resulting in a loss of electrolytes and water due to an impaired epithelial barrier and higher net secretion.

Methods: A murine small intestinal organoid model was established to study (long-term) effects of TNFα on the intestinal epithelium using live imaging, immunohistochemical staining and qPCR.

Multimodal Imaging and Management of a Metastatic Cardiac Intimal Sarcoma.

• Echocardiography is the modality of choice for initial cardiac tumor diagnosis. • CMR aids in tissue characterization, perfusion assessment, and delineating anatomy. • Intimal sarcomas are the most common primary cardiac sarcomas.

Ionotropic P2X4 and P2X7 receptors in the regulation of ion transport across rat colon.

Background And Purpose: ATP plays an important role as an extracellular messenger acting via different types of purinoceptors. Whereas most of the actions of ATP at intestinal epithelia are thought to be mediated by metabotropic P2Y receptors, the role of ionotropic P2X receptors remains unclear. Consequently, we investigated the role of P2X4 and P2X7 receptors on ion transport across rat colonic epithelia by using BzATP, a potent agonist at P2X7 (and weak agonist at P2X4).