Publications by authors named "J B Parkhurst"
Attention-deficit/hyperactivity disorder (ADHD) is a common neurodevelopmental disorder that typically presents in childhood and can cause significant impairment from youth into adulthood across multiple settings (eg, school, home). Pediatricians are the most likely health professionals to identify and treat ADHD. However, ADHD symptoms often overlap with other common mental health disorders, making accurate identification challenging.
View Article and Find Full Text PDFThis chapter describes additions to the DIALS software package for processing serial still-shot crystallographic data, and the implementation of a pipeline, xia2.ssx, for processing and merging serial crystallography data using DIALS programs. To integrate partial still-shot diffraction data, a 3D gaussian profile model was developed that can describe anisotropic spot shapes.
View Article and Find Full Text PDFConformational heterogeneity of biological macromolecules is a challenge in single-particle averaging (SPA). Current standard practice is to employ classification and filtering methods that may allow a discrete number of conformational states to be reconstructed. However, the conformation space accessible to these molecules is continuous and, therefore, explored incompletely by a small number of discrete classes.
View Article and Find Full Text PDFPillar data-acquisition strategies for cryo-electron tomography of beam-sensitive biological samples.
Acta Crystallogr D Struct Biol
June 2024
Article Synopsis
- Cryo-electron tomography (cryo-ET) often uses a planar sample geometry, but this leads to challenges like increased sample thickness and decreased signal-to-noise ratio at high tilt angles, limiting data collection.
- The typical maximum tilt range is ±70°, which results in a "missing wedge" in Fourier space that can hinder analysis of low-symmetry biological structures, such as organelles or vesicles.
- To tackle these issues, an innovative n-helix tilt scheme involving overlapping tilt series is proposed, which optimizes data acquisition from a pillar sample geometry while addressing electron beam damage and data alignment.
Background: Bright light therapy (BLT) has not been well-studied in adolescents with major depressive disorder, particularly in outpatient settings.
Methods: We conducted an 8-week clinical trial of BLT in adolescents recruited from a primary care practice with moderate to severe major depression. Acceptability and feasibility were defined by daily use of the light box and integration into daily routines.