Determination of the De Novo Minimum Selection Concentration of Trimethoprim In Vivo for Using A Pilot Study.

We investigated whether the maximum residual levels of trimethoprim permitted in food (Acceptable Daily Intake-ADI) could select for de novo trimethoprim resistance in in vivo. We designed chronic infection models of in and exposed them to sub-ADI doses of trimethoprim through a single-dosing regimen. The emergence of trimethoprim resistance was determined by isolating the target bacteria on selective agar plates, followed by species confirmation using MALDI-TOF mass spectrometry.

Fetal Cartilage Progenitor Cells in the Repair of Osteochondral Defects.

Background: Therapies for cartilage restoration are of great interest, but current options provide limited results. In salamanders, interzone (IZN) tissue can regenerate large joint lesions. The mammalian homolog to this tissue exists during fetal development and exhibits remarkable chondrogenesis in vitro.

On-surface synthesis.

is pleased to introduce a Collection of research works focused on recent developments within the interdisciplinary field of on-surface synthesis. Here, the Guest Editors highlight key themes and look towards the future of this research field.

IOC consensus statement: interpersonal violence and safeguarding in sport.

Objective: Interpersonal violence (IV) in sport is challenging to define, prevent and remedy due to its subjectivity and complexity. The 2024 International Olympic Committee Consensus on Interpersonal Violence and Safeguarding aimed to synthesise evidence on IV and safeguarding in sport, introduce a new conceptual model of IV in sport and offer more accessible safeguarding guidance to all within the sports ecosystem by merging evidence with insights from Olympic athletes.

Methods: A 15-member expert panel performed a scoping review following Joanna Briggs Institute methodologies.

Exfoliation of Graphene onto Si(111)-7 × 7 under Ultrahigh Vacuum Provides Some Protection against Exposure to Air.

The investigation of graphene as a protective coating for different materials has been an area of active research for well over a decade. However, graphene's ability to protect clean, reconstructed silicon from ambient gases has remained uninvestigated. Here, we describe the use of a clean ultrahigh vacuum transfer method to deposit graphene onto the Si(111)-7 × 7 reconstruction.