Nanopore sequencing to detect A-to-I editing sites.

Adenosine-to-inosine (A-to-I) RNA editing, mediated by the ADAR family of enzymes, is pervasive in metazoans and functions as an important mechanism to diversify the proteome and control gene expression. Over the years, there have been multiple efforts to comprehensively map the editing landscape in different organisms and in different disease states. As inosine (I) is recognized largely as guanosine (G) by cellular machineries including the reverse transcriptase, editing sites can be detected as A-to-G changes during sequencing of complementary DNA (cDNA).

The role of lncRNA NEAT1 in acute graft-versus-host disease: Regulation of macrophage polarization and inflammatory cytokine secretion via JNK/NLRP3 pathway.

Background: Acute graft-versus-host disease (aGVHD) is a complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT). The role of macrophages as proficient antigen-presenting cells in aGVHD is a prominent area of investigation in contemporary research. The association between long noncoding RNA nuclear enriched abundant transcript 1 (lncRNA NEAT1) and the macrophage function is of significant interest.

Identification of EXO1 as a potential biomarker associated with prognosis and tumor immune microenvironment for specific human cancers.

Exonuclease 1 (EXO1) is an evolutionarily conserved exonuclease, which have function on maintaining genomic stability. Elevated expression of EXO1 has been reported in certain cancers. However, a comprehensive pan-cancer analysis of EXO1 is still lacking and its role in human cancer development remains poorly understood.

Effect of connective tissue growth factor and inflammatory factors on the condition and prognosis of patients undergoing reperfusion for acute ischemic stroke.

Objective: To evaluate the impact of connective tissue growth factor (CTGF) and inflammatory factors on the condition and prognosis of patients undergoing reperfusion therapy for acute ischemic stroke (AIS).

Methods: A retrospective analysis was conducted on 212 AIS patients who received reperfusion therapy at Wu Xi Traditional Chinese Medicine Hospital, Suqian Hospital of Traditional Chinese Medicine, The Affiliated Wuxi People's Hospital of Nanjing Medical University from January 2021 to January 2024. Patients were divided into a control group (modified Rankin Scale [mRS] score = 0-3, n = 132) and a study group (mRS score = 4-6, n = 80).