Annotation of cis-regulatory-associated histone modifications in the genomes of two Thoroughbred stallions.

The Functional Annotation of Animal Genomes (FAANG) consortium aims to annotate animal genomes across species, and work in the horse has substantially contributed to that goal. As part of this initiative, chromatin immunoprecipitation with sequencing (ChIP-seq) was performed to identify histone modifications corresponding to enhancers (H3K4me1), promoters (H3K4me3), activators (H3K27ac), and repressors (H3K27me3) in eight tissues from two Thoroughbred stallions: adipose, parietal cortex, heart, lamina, liver, lung, skeletal muscle, and testis. The average genome coverage of peaks identified by MACS2 for H3K4me1, H3K4me3, and H3K27ac was 6.

Self-Perception as a Predictor of Eating Disorder Severity in a Residential Sample of Women and Girls With Eating Disorders.

Eating disorders are serious mental health conditions with significant negative health outcomes, high mortality rates, and comorbid mental health conditions. Despite many available interventions for eating disorders, treatment remains challenging due to the difficulty in maintaining treatment gains. Understanding effective treatment processes is crucial.

Recognizing individual variability in misophonia: Identifying symptom-based subgroups with Gaussian mixture modeling.

Misophonia is characterized by intense emotional and physiological reactions to everyday sounds such as chewing and tapping. While previous researchers have focused on defining and characterizing the disorder, limited attention has been given to the variability in symptom presentations across individuals. In this study, we sought to identify distinct subgroups of individuals with misophonia by applying a Gaussian finite mixture model to explore the heterogeneity of symptom profiles.

Prenatal exposure to organochlorine pesticides and polychlorinated biphenyls and risk of testicular germ cell cancer later in life.

Introduction: Exposure to environmental chemicals during fetal development may increase the risk of testicular germ cell cancer (TGCC), but few studies have tested the hypothesis. We focused on organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs), previously investigated in relation to other male reproductive health outcomes.

Methods: We conducted a nested case-control study of 332 mother-son pairs, comprising 65 TGCC cases and 267 controls, identified from a Danish Pregnancy Screening Registry with biobanked serum samples collected from pregnant women in 1985-1995, when exposure to the studied chemicals was relatively high.

AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates.

The nutrient-sensitive protein kinases AMPK and mTORC1 form a fundamental negative feedback loop that governs cell growth and proliferation. mTORC1 phosphorylates α2-S345 in the AMPK αβγ heterotrimer to suppress its activity and promote cell proliferation under nutrient stress conditions. Whether AMPK contains other functional mTORC1 substrates is unknown.