Risk factors for complications in total hip arthroplasty.

Introduction And Objective: Hip arthroplasty represents a significant advancement in the treatment of refractory chronic joint pain, improving quality of life and functionality. The objective of this study is to identify the risk factors associated with local and systemic complications in patients treated with total hip arthroplasty.

Methods: Observational, analytical, retrospective cohort study, which included 304 participants treated with total hip replacement.

Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells.

Human induced pluripotent stem cells (hiPSCs) are capable of unlimited proliferation and can differentiate in vitro to generate derivatives of the three primary germ layers. Genetic and epigenetic abnormalities have been reported by Wissing and colleagues to occur during hiPSC derivation, including mobilization of engineered LINE-1 (L1) retrotransposons. However, incidence and functional impact of endogenous retrotransposition in hiPSCs are yet to be established.

Development of a spectrofluorimetric method for the determination of ofloxacin in urine.

A simple, accurate, rapid, and sensitive spectrofluorimetric method for the determination of ofloxacin in urine was developed by means of first derivative matrix isopotential synchronous fluorescence spectrometry (MISF). The calibration curve was found to be linear in the concentration range 40-320 ng/mL. The method allows the determination of compound in samples with unknown background fluorescence without the need for tedious pre-separation.

Simultaneous determination of mefenamic and tolfenamic acids in real samples by terbium-sensitized luminescence.

A simple luminescent methodology for the simultaneous determination of mefenamic and tolfenamic acids in pharmaceutical preparations and human urine is proposed. Since the native fluorescence of both analytes is not intense, the method takes advantage of the lanthanide-sensitized luminescence, which provides a higher sensitivity. Due to the strong overlapping between the luminescence spectra of both terbium complexes, the use of luminescence decay curves to resolve mixtures of the analytes is proposed, since these curves are more selective.