Publications by authors named "J A Sellers"
Background: Point of need diagnostics provide efficient testing capability for remote or austere locations, decreasing the time to answer by minimizing travel or sample transport requirements. Loop-mediated isothermal amplification (LAMP) is an appealing technology for point-of-need diagnostics due to its rapid analysis time and minimal instrumentation requirements.
Methods: Here, we designed and optimized nine LAMP assays that are sensitive and specific to targeted bacterial select agents including Bacillus anthracis, Francisella tularensis, Yersinia pestis, and Brucella spp.
Purpose: Pigment epithelium-derived factor (PEDF) is a neurotrophic glycoprotein secreted by the retinal pigment epithelium (RPE) that supports retinal photoreceptor health. Deficits in PEDF are associated with increased inflammation and retinal degeneration in aging and diabetic retinopathy. We hypothesized that light-induced stress in C57BL/6J mice deficient in PEDF would lead to increased retinal neuronal and RPE defects, impaired expression of neurotrophic factor Insulin-like growth factor 1 (IGF-1), and overactivation of Galectin-3-mediated inflammatory signaling.
View Article and Find Full Text PDFPurpose: The aim of this study was to test whether oral administration of nicotinamide riboside (NR), the nicotinamide adenine dinucleotide (NAD+) precursors, protect retina ganglion cells (RGCs) from neurodegeneration in DBA/2J (D2) mice, which is a widely used mouse model of age-related inherited glaucoma.
Method: Oral NR or NAM administration (NR low dose: 1150mg/kg; NR high dose: 4200mg/kg; NAM low dose group: 500mg/kg; NAM high dose: 2000mg/kg of body weight per day) essentially started when D2 mice were 4 or 9 months old and continued up to 12 months old. Control cohort identically received food/water without NAM or NR.
The forced-response method: A new chronometric approach to measure conflict processing.
Behav Res Methods
December 2024
Article Synopsis
- Free reaction times (RTs) are commonly used in cognitive psychology for measuring conflict resolution, despite ongoing concerns about their effectiveness.
- The report introduces a new approach called the forced-response method to study speed-accuracy trade-off functions (SATs) in conflict tasks, using an arrow flanker task as an example.
- This method reveals a detailed time course of flanker interference effects and shows that manipulations affecting free RT paradigms also influence accuracy in forced-response tasks, highlighting the method's relevance and potential for deeper insights into cognitive processing dynamics.
Article Synopsis
- Non-muscle myosin II (NMII) plays a crucial role in biological processes, but current therapeutic options are limited due to non-selective inhibitors like blebbistatin that affect both NMII and cardiac myosin II (CMII).
- Researchers developed a series of selective NMII inhibitors, notably MT-228, which demonstrates high brain penetration and effectiveness in preclinical models for stimulant use disorder, a condition lacking FDA-approved treatments.
- The structure of MT-228 binding to myosin II reveals its 17-fold selectivity for NMII over CMII, providing insights for future drug development and potential applications in various medical fields, including cancer and nerve regeneration.