A Prototype Process for Demulsification of Waste Ice Cream.

Recovery of the butterfat in waste ice cream may be an opportunity to mitigate food and economic loss. Previous efforts to recover such fat have succeeded in producing a fat-enriched fraction but have not succeeded in demulsifying the fat. In the present study, a method involving a sequence of emulsion-breaking steps is shown to be effective for releasing a majority of the fat from waste ice cream as free, unemulsified oil.

Integrating Bacteriocins and Biofilm-Degrading Enzymes to Eliminate Persistence.

is a Gram-positive bacterium causing listeriosis, a severe infection responsible for significant morbidity and mortality globally. Its persistence on food processing surfaces via biofilm formation presents a major challenge, as conventional sanitizers and antimicrobials exhibit limited efficacy against biofilm-embedded cells. This study investigates a novel approach combining an engineered polysaccharide-degrading enzyme (CAase) with a bacteriocin (thermophilin 110) produced by .

Galacto-oligosaccharide production by ssp. whole cells and lysates.

β-Galactosidase is currently applied in foods for reduction of lactose but can also be used for its transgalactosylation activity to synthesize galacto-oligosaccharides (GOS) as prebiotics. The ability of GRAS-status strains to exhibit such activities would benefit consumers given their extensive history with dairy products. The objective of this study was to characterize the production of GOS in 6 strains for their ability to synthesize GOS in 50 m sodium phosphate (pH 6.

BlpU is a broad-spectrum bacteriocin in .

strain B59671 naturally produces thermophilin 110, a broad-spectrum bacteriocin encoded within the bacteriocin-like peptide () gene cluster, and thermophilin 13 from a separate chromosomal locus. Analysis of the gene cluster revealed two genes, and , as potentially encoding bacteriocins. Deletion of from the B59671 chromosome did not result in a loss of antimicrobial activity against either ST113 or F.

The quorum sensing peptide BlpC regulates the transcription of genes outside its associated gene cluster and impacts the growth of .

Bacteriocin production in is regulated by cell density-dependent signaling molecules, including BlpC, which regulates transcription from within the bacteriocin-like peptide () gene cluster. In some strains, such as ST106, this signaling system does not function properly, and BlpC must be supplied exogenously to induce bacteriocin production. In other strains, such as B59671, bacteriocin (thermophilin 110 in strain B59671) production occurs naturally.