Modeling wood product carbon flows in southern us pine plantations: implications for carbon storage.

Background: Wood products continue to store carbon sequestered in forests after harvest and therefore play an important role in the total carbon storage associated with the forest sector. Trade-offs between carbon sequestration/storage in wood product pools and managed forest systems exist, and in order for forest sector carbon modeling to be meaningful, it must link wood product carbon with the specific forest system from which the products originate and have the ability to incorporate in situ and ex situ carbon synchronously over time.

Results: This study uses elements of a life cycle assessment approach, tracing carbon from US southern pine timber harvests to emission, to create a decision support tool that practitioners can use to inform policy design around land- and bioproduct-based mitigation strategies.

Following NCCN guidelines within one hospital system in the United States: Comparison between cancer centers and genetic counselor utilization.

Genetic testing is an instrumental tool used to determine whether an individual has a predisposition to certain cancers. Knowing of a hereditary cancer predisposition may allow a patient and their family to consider high-risk screening or risk-reducing options. Genetic counselors work with physicians to identify patients at increased risk for genetic testing using available guidelines such as those provided by the National Comprehensive Cancer Network (NCCN).

Secretion-and-capture cell-surface display for selection of target-binding proteins.

This report describes a new cell-surface display system, the Secretion and Capture Technology (SECANT™) platform, which relies on in vivo biotinylation of the protein of interest followed by its capture on the avidinated surface of the parent cell. Cell sorting techniques are then used to isolate clones that display target-binding protein. A distinguishing feature of this method is its ability to display complex proteins, such as full-length immunoglobulin G (IgG) antibodies, on living cells.

Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies in Saccharomyces cerevisiae.

Because of its eukaryotic nature, simple fermentation requirements, and pliable genetics, there have been many attempts at improving recombinant protein production in Saccharomyces cerevisiae. These strategies typically involve altering the expression of a native protein thought to be involved in heterologous protein trafficking. Usually, these approaches yield three- to tenfold improvements over wild-type strains and are almost always specific to one type of protein.

A flow cytometric assay for screening improved heterologous protein secretion in yeast.

Selection of highly productive hosts for protein expression is a significant component of bioprocess design. As an alternative to traditional plate, halo, and suppression-based screens, we describe a high-throughput, flow cytometric assay, the Cell Surface Secretion Assay (CeSSA), that can be used to select for improved heterologous protein secretion from a population of S. cerevisiae mutants.