Development and Multicenter Case-Control Validation of Urinary Comprehensive Genomic Profiling for Urothelial Carcinoma Diagnosis, Surveillance, and Risk-Prediction.

Purpose: Urinary comprehensive genomic profiling (uCGP) uses next-generation sequencing to identify mutations associated with urothelial carcinoma and has the potential to improve patient outcomes by noninvasively diagnosing disease, predicting grade and stage, and estimating recurrence risk.

Experimental Design: This is a multicenter case-control study using banked urine specimens collected from patients undergoing initial diagnosis/hematuria workup or urothelial carcinoma surveillance. A total of 581 samples were analyzed by uCGP: 333 for disease classification and grading algorithm development, and 248 for blinded validation.

Chronic renal failure and bladder augmentation: stomach versus sigmoid colon in the canine model.

A canine model for chronic renal failure and bladder augmentation with a segment of stomach or sigmoid colon (or control) was developed to evaluate the possible benefit of net chloride ion excretion noted in an earlier canine model for gastrocystoplasty. Gastrocystoplasty animals behaved similar to control animals relative to the sigmoid cystoplasty animals, tolerated significantly greater acid loading, did not have hyperchloremia with acidosis and had less weight loss with acidosis. Gastrocystoplasty seems to be superior to sigmoid cystoplasty in the azotemic canine.

Gastrocystoplasty and colocystoplasty in canines: the metabolic consequences of acute saline and acid loading.

An experimental canine model was developed to quantitate the net transmural ion flux of colon and gastric segments used for augmentation cystoplasty. Gastrocystoplasty (4) or colocystoplasty (4) was constructed in 8 female mongrels. Acute intravenous saline and acid loading experiments were performed 2 to 4 weeks after cystoplasty.

Intestinocystoplasty and total bladder replacement in children and young adults: followup in 129 cases.

A review of 129 consecutive young patients (average age 13.4 years) who underwent intestinocystoplasty or total bladder replacement during a 6 1/2-year period at our institution is presented. The most common diagnosis was myelodysplasia and the average followup was 44 months.

Indiana continent urinary reservoir.

Cecoileal reservoirs were created in 29 patients. Tunneled ureteral implantations along the tenia of the cecum provided the antireflux mechanism. Plication or tapering of the terminal ileal segment along with the ileocecal valve provided the continence mechanism.