Application of topological resonances in experimental investigation of a Fermi golden rule in microwave networks.

We investigate experimentally a Fermi golden rule in two-edge and five-edge microwave networks with preserved time reversal invariance. A Fermi golden rule gives rates of decay of states obtained by perturbing embedded eigenvalues of graphs and networks. We show that the embedded eigenvalues are connected with the topological resonances of the analyzed systems and we find the trajectories of the topological resonances on the complex plane.

Non-Weyl Microwave Graphs.

One of the most important characteristics of a quantum graph is the average density of resonances, ρ=(L/π), where L denotes the length of the graph. This is a very robust measure. It does not depend on the number of vertices in a graph and holds also for most of the boundary conditions at the vertices.

Use and Misuse of Opioids in Maine: Results From Pharmacists, the Prescription Monitoring, and the Diversion Alert Programs.

Objective: Although opioids have substantial efficacy for acute pain management, escalation to opioid misuse and abuse is a persistent concern. This report assesses the current status of the opioid epidemic in Maine using three complementary data sets.

Method: A representative sample of pharmacists (N = 275) completed an online survey regarding the extent that opioids affected their practice.

Rapid flow cytometric method for viability determination of solventogenic clostridia.

We endeavored to develop a method for viability determination of solventogenic clostridia and to apply it for monitoring acetone-butanol-ethanol (ABE) fermentation. Six fluorescent probes (propidium iodide [PI], ethidium bromide, fluorescein diacetate, carboxyfluorescein diacetate [cFDA], rhodamine 123, bis-(1,3-dibutylbarbituric acid)trimethine oxonol [BOX]) were tested in order to distinguish two subpopulations of live and dead clostridial cells in suspension. Three of them were found to be appropriate (PI, BOX and cFDA) for this purpose.

Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum.

Clostridium pasteurianum forms acetic and butyric acids in an initial growth phase, which is a typical feature of clostridial acetone-butanol fermentation where an initial accumulation of acids is followed by production of solvents 1-butanol, acetone and ethanol. The initiation of the solvent production coupled with endospore formation leads to decrease of cell-wall thickness; thinner cell wall is more resistant against solvents and dyes. These changes can be observed by the method based on adaptation of Gram staining.