Development of Heterodera glycines Ichinohe on Soybean, Glycine max (L.) Merr., under Gnotobiotic Conditions.

The life cycle of the soybean cyst nematode, Race 3 (SCN 3), Heterodera glycines Inchinohe was determined from observations of the developmental stages on soybean Glycine max cv. Kent root explants under gnotobiotic conditions at 25 C. Approximately 51% of the second-stage larvae penetrated the root l day after inoculation (DAI).

Life Cycle of Heterodera zeae Koshy, Swarup, and Sethi on Zea mays L. Axenic Root Explants.

Monoxenic cultures of Heterodera zeae, the corn cyst nematode (CCN), were established on root explants of corn Zea mays L., cv. Kenworthy.

Screening Soybean for Resistance to Heterodera glycines Ichinohe Using Monoxenic Cultures.

A simple, rapid, and inexpensive method for evaluation of host-parasite interactions, based on monoxenic cultures, is described. Axenic root explants of Glycine max (L.) Merr.