Glycogenesis and glyconeogenesis from glutamine, lactate and glycerol support human macrophage functions.

Macrophages fight infection and ensure tissue repair, often operating at nutrient-poor wound sites. We investigated the ability of human macrophages to metabolize glycogen. We observed that the cytokines GM-CSF and M-CSF plus IL-4 induced glycogenesis and the accumulation of glycogen by monocyte-derived macrophages.

Nitrogen nutrition effects on δC of plant respired CO are mostly caused by concurrent changes in organic acid utilisation and remobilisation.

Nitrogen (N) nutrition impacts on primary carbon metabolism and can lead to changes in δC of respired CO. However, uncertainty remains as to whether (1) the effect of N nutrition is observed in all species, (2) N source also impacts on respired CO in roots and (3) a metabolic model can be constructed to predict δC of respired CO under different N sources. Here, we carried out isotopic measurements of respired CO and various metabolites using two species (spinach, French bean) grown under different NH :NO ratios.

Combining Gas Exchange and Rapid Quenching of Leaf Tissue for Mass Spectrometry and NMR Analysis Using an External Chamber.

We describe here a method to study and manipulate photorespiration in intact illuminated leaves. When the CO/O mole fraction ratio changes, instant sampling is critical, to quench leaf metabolism and thus trace rapid metabolic modification due to gaseous conditions. To do so, we combine CO labeling and gas exchange, using a large custom leaf chamber to facilitate fast sampling by direct liquid nitrogen spraying.

Return to Running after Knee Arthroplasty: A Case Report.

Individuals who have undergone knee arthroplasty may still want to run, but no study has reported a progression to guide patients and clinicians. The objective of this case report is to document the process of returning to running after total knee arthroplasty with a 1-year follow-up. The client was a 55-year-old woman, former triathlete, who underwent unilateral knee arthroplasty 1 year prior to consultation.