Publications by authors named "J A Grossmann"
Mass spectrometry is a cornerstone of quantitative proteomics, enabling relative protein quantification and differential expression analysis () of proteins. As experiments grow in complexity, involving more samples, groups, and identified proteins, interactive differential expression analysis tools become impractical. The addresses this challenge by providing a command-line interface that simplifies , making it accessible to nonprogrammers and seamlessly integrating it into workflow management systems.
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- The protocol outlines a method for assessing the fitness of the oral symbiont A. aphrophilus related to its type VI secretion system (T6SS) using competition assays and metaproteomics.
- It includes guidelines for designing T6SS-specific mutants and conducting competition tests with the pathobiont A. actinomycetemcomitans in biofilm models.
- The biofilm model simulates the oral plaque ecosystem, featuring seven species, and for detailed procedures, it refers to the work of Oscarsson et al.
It has remained yet unclear which soluble factors regulate the anti-inflammatory macrophage phenotype observed in both homeostasis and tumourigenesis. We show here that haptoglobin, a major serum protein with elusive immunoregulatory properties, binds and buffers bacterial lipopolysaccharides to attenuate activation of NFκB in macrophages. Haptoglobin binds different lipopolysaccharides with low micromolar affinities.
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- Assisted reproductive technologies in equine reproduction show low success with conventional IVF, emphasizing the importance of the 'cumulome' related to oocyte development.
- A total of 1671 proteins and 612 metabolites were analyzed from cumulus-oocyte complexes, comparing immature and matured oocytes through various stages of development.
- Findings indicate that the mature oocyte groups have enhanced energy metabolism and vesicular transport pathways, while immature oocytes show different protein profiles related to extracellular matrix composition and lower ATP-related compounds.
Article Synopsis
- Miyoshi myopathy/dysferlinopathy (MMD) is a rare muscle disease linked to mutations in the DYSF gene, which also affects the brain's structure and function, although this impact hasn't been fully studied.
- Using MRI techniques, a family study revealed significant differences in the brain of MMD patients, including an asymmetrical increase in the size of their inferior lateral ventricles and notable decreases in magnesium levels and energy metabolism in brain regions like the hippocampus.
- Following a month of magnesium supplementation, the MMD patients showed improvements, suggesting potential benefits and calling for more research into the brain functions of MMD patients and further testing of magnesium therapy.